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Food Science and Human Nutrition Department

Food Science and Human Nutrition Department

Dr. James F. Collins

Professor & FSHN Graduate Committee Chair

Programs/Background

  • Research

    Research:

    Dr. Collins’ research interests include molecular mechanisms of intestinal iron transport, the influence of copper on iron homeostasis, nutrient-gene interactions (i.e. nutrigenomics), nutrient control of gene transcription, and iron absorption during inflammatory states (e.g. anemia of chronic disease).

    Publications:

    1. Wang, X., Garrick, M.D., Collins, J.F. (2019) Animal Models of Normal and Disturbed Iron and Copper HomeostasisInvited Critical Review. J. Nutr. 149(12):2085-2100
    2. Wang, X., Zhang, M. Flores, S.R.L., Woloshun, R.R., Wang, Yang, C., L. Xiang, P., Xu, X., Garrick, M.D., Vidyasagar, S., Merlin, D., Collins, J.F. (2019) Oral Gavage of Ginger Nanoparticle-derived Lipid Vectors Loaded with Dmt1 siRNA Mitigates Iron Loading in Murine Hereditary Hemochromatosis. Mol. Ther. 27(3):493-506.
    3. Doguer, C., Ha, J-H., Collins, J.F. (2018) Intersection of Iron and Copper Metabolism in the Mammalian Intestine and Liver. Comp. Physiol. 14;8(4):1433-1461, 2018.
    4. Wang, X., Flores, S.R.L., Ha, J-H., Doguer, C., Woloshun, R.R, Xiang, P., Grosche, A., Vidyasagar, S., Collins, J.F. (2018) Intestinal Divalent Metal-ion Transporter 1 (DMT1) is Essential for Optimal Assimilation of Dietary Copper in Male and Female Mice With Iron-Deficiency Anemia. J. Nutr. 148(8):1244-1252, 2018.
    5. Wang, T., Xiang, P., Ha, J-H., Wang, X., Doguer, C., Flores, S.R.L., Kang, Y.J., Collins, J.F. (2018) Copper supplementation reverses dietary iron overload-induced pathologies in mice. J. Nutr. Biochem. 59:56-63, 2018.
    6. Ha, J-H, Doguer, C., Flores, S.R.L., Wang, T., Collins, J.F. (2018) Progressive Increases in Dietary Iron are Associated with the Emergence of Pathologic Disturbances of Copper Homeostasis in Growing Rats. J. Nutr. 148(3): 373-378.
    7. Collins, J.F. (2018) Ferroxidases and Mammalian Iron Homeostasis: Novel Insight into a Physiological Phenomenon First Described More Than Half a Century Ago. Editorial Comment. Cell. Mol. Gastroenterol. Hepatol. 6(4):470-471.
    8. Doguer, C., Ha, J-H, Gulec, S., Vuple, C.D., Anderson, G.J., Collins, J.F. (2017) Intestinal Hephaestin Potentiates Iron Absorption In Weanling, Adult and Pregnant Mice Under Physiological Conditions. Blood Adv. 1:1335-1346.
    9. Ha, J-H., Doguer, C., Collins, J.F. (2017) Consumption of a High-Iron Diet Disrupts Homeostatic Regulation of Intestinal Copper Absorption in Adolescent MiceAm. J. Physiol. GIL Physiol. 313: G353-G360.
    10. Zhang, M., Wang, X., Han, M.K., Collins, J.F., Merlin, D. (2017) Oral administration of ginger-derived nanolipids loaded with siRNA as a novel approach for efficient siRNA drug delivery to treat ulcerative colitis. Nanomedicine (Lond). 12(16): 1927-1943.
    11. Ha, J-H., Doguer, C., Wang, X., Flores, S.R., Collins, J.F. (2016) High-iron consumption impairs growth and causes copper-deficiency anemia in weanling Sprague-Dawley rats. PLoS One. 11(8):e0161033.
    12. Ha, J-H., Doguer, C. Collins, J.F. (2016) Knockdown of copper-transporting ATPase 1 (Atp7a) impairs iron flux in fully-differentiated rat (IEC-6) and human (Caco-2) intestinal epithelial cells. Metallomics. 8(9):963-972.
    13. Zhang, M., Collins, J.F., Merlin, D. (2016) Do ginger-derived nanoparticles represent an attractive treatment strategy for inflammatory bowel diseases? Nanomedicine (Lond). 11(23)3035-3037.
    14. Collins, J.F. (2015) Long noncoding RNAs and hepatocellular carcinoma. Editorial Comment. Gastroenterology. 148(2): 291-94.
    15. Gulec, S., Anderson, G.J., Collins, J.F. (2014) Mechanistic aspects of intestinal iron transport.  Am. J. Physiol. GIL Physiol. 307(4): G397-409.
    16. Gulec, S., Collins, J.F. (2014) Molecular mediators governing iron-copper interactions. Ann. Rev. Nutr. 34: 95-116.
    17. Gulec, S., Collins, J.F. (2014) Silencing the Menkes copper transporting ATPase (Atp7a) gene in rat intestinal epithelial cells increases iron flux via transcriptional induction of ferroportin 1 (Fpn1). J. Nutr. 144(2): 12-9.
    18. Gulec, S., and Collins, J.F.  (2014) Silencing of the Menkes copper-transporting ATPase (Atp7a) gene increases cyclin D1 protein expression and impairs proliferation of rat intestinal epithelial (IEC-6) cells. J. Trace. Elem. Med. Biol. 28(4): 459-64.
    19. Jiang, L., Garrick, M.D., Garrick, L.M. Zhao, L., Collins, J.F. (2013) Divalent metal transporter 1 (Dmt1) mediates copper transport in the duodenum of iron-deficient rats and when overexpressed in iron-deprived HEK-293 cells. J. Nutr. 143(12):1927-33.
    20. Xie, L., Collins, J.F. (2013) Transcription factors Sp1 and Hif2α mediate induction of the copper-transporting ATPase (Atp7a) gene in intestinal epithelial cells during hypoxia. J. Biol. Chem. 288(33):23943-52.
    21. Gulec, S., Collins, J.F. (2013) Investigation of iron metabolism in mice expressing a mutant Menkes copper transporting ATPase (Atp7a) protein with diminished activity (Brindled; MoBr/y).  PLOS One. 11;8(6):e66010.
    22. Xie, L., Collins, J.F. (2012) Copper stabilizes the Menkes copper-transporting ATPase (Atp7a) protein in rat intestinal epithelial cells. Am. J. Physiol. Cell Physiol., 304(3):C257-62
    23. Lu, Y., Kim, C., Collins J.F. (2012) Multiple Menkes copper ATPase (Atp7a) transcript and protein variants are induced by iron deficiency in rat duodenal enterocytes.  J. Trace Elem. Biol. Med. 26(2-3):109-14
    24. Ranganathan, P.N., Lu, Y., Fuqua, B.K., Collins, J.F. (2012) Discovery of a cytosolic/soluble ferroxidase in rodent enterocytes. Proc. Natl. Acad. Sci. USA. 109(9):3564-9.
    25. Ranganathan, P.N., Lu, Y., Fuqua, B.K., Collins, J.F. (2012) Immunoreactive Hephaestin and ferroxidase activity are present in the cytosolic fraction of rat enterocytes. Biometals. 25(4):687-95.
    26. Klevay, L., Collins, J.F. (2011) Copper. Adv. Nutr. 2(6):520-22.
    27. Jiang, L, Ranganathan, P.N., Lu, Y., Kim, C., Collins J.F. (2011) Exploration of the Copper Related Compensatory Response in the Belgrade Rat Model of Genetic Iron Deficiency. Am. J. Physiol. Gastrointest. Liver Physiol. 301(5):G877-86.
    28. Ranganathan, P.N., Lu, Y., Jiang, L., Kim, C. Collins, J.F. (2011) Serum ceruloplasmin protein expression and activity increases in iron-deficient rats and is further enhanced by higher dietary copper intake. Blood. 118(11):3146-53.
    29. Xie, L., Collins, J.F.  (2011) Transcriptional regulation of the Menkes copper ATPase (Atp7a) gene by hypoxia-inducible factor (HIF2α) in intestinal epithelial cells. Am. J. Physiol. Cell Physiol. 300(6):C1298-305.
    30. Hu, Zihua, Gulec, S., Collins, J.F. (2010) Cross-Species Comparison of Genome-Wide Gene Expression Profiles Reveals a Preferential Induction of Hypoxia Inducible Factor (HIF) Responsive Genes in Iron Deprived Intestinal Epithelial Cells. Am. J. Physiol. Cell Physiol. 299:930-938.
    31. 31.    Collins, J.F., Prohaska, J.R., Knutson, M.K. (2010) Metabolic Crossroads of Iron and Copper. Nutr. Rev. 68(3):133–147.

    Textbook Chapters:

    1.       Collins, J. F. (2019) “Copper”. In: Present Knowledge in Nutrition, 11th edition. Wiley. Under review. 

    2.       Anderson G.J., Lu Y., Frazer D. M., Collins, J.F. (2019) “Intestinal Iron Absorption”. In: Encyclopedia of Gastroenterology. 2nd edition. Academic Press.

    3.       Collins, J.F. (2017) “Copper: Basic Physiologic and Nutritional Aspects”. In: Molecular, Genetic and Nutritional Aspects of Major and Trace Minerals. Academic Press

    4.       Collins, J.F., Flores, S.R., Wang, X. and Anderson, G.J. (2017) “Mechanisms of Intestinal Iron Absorption” In: Physiology of the Gastrointestinal Tract, 6th Edition. Academic Press.

    5.       Collins, J.F. (2011) “Copper” for Modern Nutrition in Health and Disease, 11th Edition; Lipcott, Williams and Wilkins.

    6.       Kiela, P.R., Collins, J.F., Ghishan, F.K. (2011) “Molecular Mechanisms of Intestinal Transport of Calcium, Phosphate and Magnesium” for Physiology of the Gastrointestinal Tract, 5th Edition. Academic Press.

    7.       Collins, J. F. and Anderson, G.J. (2011) “Mechanisms of Intestinal Iron Absorption” for Physiology of the Gastrointestinal Tract, 5th Edition. Academic Press.

    8.       Collins, J.F. Bai, L., Xu, H., and Ghishan, F.K. (2005) “Molecular Aspects and Regulation of Gastrointestinal Function During Post-Natal Development”. For Physiology of the Gastrointestinal Tract, 4th Edition. Academic Press.

    9.       Collins, J.F. and Ghishan, F.K. (2005) “Molecular Mechanisms of Intestinal Transport of Calcium, Phosphate and Magnesium”. For Physiology of the Gastrointestinal Tract, 4th Edition. Academic Press.

  • Additional Research Publications
    1. Wang, X., Zhang, M. Woloshun, R.R., Yu, Y., Lee, J.K., Flores, S. R. L., Merlin, D., Collins, J.F. (2021) siRNA-mediated Knockdown of Intestinal Dmt1 Potentiates the Iron-lowering Effect of Dietary Iron Restriction in Hamp KO Mice. Submitted. 
    2. Flores, S.R.L, Nelson, S., Woloshun, R.R., Wang, X., Ha, J-H., Yu, Y., Merlin, D., Collins, J.F. (2021) Intestinal iron absorption is appropriately modulated to match physiological demand for iron in wild-type and iron-loaded Hamp (hepcidin) knockout rats during acute colitis. PLOS One. In Revision. 
    3. Lee, J.K., Ha, J-H., Collins, J.F. (2021) Dietary Iron Intake in Excess of Requirements Impairs Intestinal Copper Absorption in Sprague-Dawley Rat Dams, Causing Copper Deficiency in Suckling Pups. Biomedicines. 9, 338. https://www.mdpi.com/2227-9059/9/4/338  
    4. Collins, J.F. (2021) Iron Chelates Hitch a Ride on PAT1. Editorial Highlight. J. Biol. Chem. 296:100418. 
    5. Sung, J., Yang, C., Collins, J. F. and Merlin, D. (2020). Preparation and Characterization of Ginger Lipid-derived Nanoparticles for Colon-targeted siRNA Delivery. Bio-protocol 10(14): e3685. DOI: 10.21769/BioProtoc.3685. 
    6. Garrick, M.D., Garrick, L. M., Zhao, L., Collins, J.F., Soukup, J., Ghio, A. J. (2019) A direct comparison of divalent metal-ion transporter 1 (DMT1) and hinokitiol, a potential small molecule replacement. Biometals. 32(5): 745-755. 
  • Teaching

    Courses Taught

    Undergraduate
    • HUN4221 Nutrition and Metabolism
    Graduate
    • HUN6356 Minerals of Nutrition
    • HUN6305 Nutritional Aspects of Carbohydrates
  • Education
    • Post Doc. University of Arizona; Transport Physiology, 1997
    • Ph.D. Vanderbilt University; Molecular Physiology, 1994
    • M.S. Middle Tennessee State University; Molecular Biology, 1991
    • B.S. University of the South (Sewanee); Biology, 1987
  • Publications
  • Lab Information

Contact Information

572 Newell Dr.
FSHN Building
Room 441A
P.O. Box 110370
Gainesville, FL 32611-0370

Phone: (352) 294-3749
Emailjfcollins@ufl.edu
Websitehttp://fshn.ifas.ufl.edu/labs/collins-lab
CV: JFC Full C.V. Updated Feb 2021
2020 UF Institute of Food & Agricultural Sciences Award