James F. Collins, Ph.D.
Education
B.S., Biology, University of the South (Sewanee), 1987
M.S., Molecular Biology, Middle Tennessee State University, 1991
Ph.D., Molecular Physiology, Vanderbilt University, 1994
Research Interests
My research program is founded in observations made by novel DNA microarray studies performed with samples isolated from the intestines of control and iron-deficient rats at different stages of post-natal development. This investigation led to the discovery that absorption of other metals is perturbed in the iron-deficient state, including increased intestinal copper transport. Subsequent studies found that the Menkes copper ATPase (ATP7A; a copper transport protein) is strongly induced by iron-deprivation and that the protein is present on both domains of intestinal epithelial cells. Other studies are underway with human duodenal biopsy samples, in collaboration with the University of Washington in Seattle. The overall objective of these studies is to identify novel genes involved in intestinal metal ion absorption in humans, by utilizing gene chip technology and other experimental methodologies. These studies are currently funded by a research grant from the National Institutes of Health.
Classes Taught
HUN2201 - Fundamentals of Human Nutrition
Selected Publications
PEER REVIEWED PUBLICATIONS
Collins, J.F., Prohaska, J.R., Knutson, M.K. (2010) Metabolic Crossroads of Iron and Copper. Nutr. Rev.68(3):133–147.
Collins J.F., Hua, P., Lu, Y., Ranganathan, P.N. (2009) Alternative Splicing of the Menkes Copper Atpase (Atp7a) Transcript in the Rat Intestinal Epithelium. Am. J. Physiol. Gastrointest. Liver Physiol. 297: G695–G707.
Collins, J.F., Wessling-Resnick, M., Knutson, M.D. (2008) Hepcidin regulation of iron transport. J. Nutrition. 138(11):2284-8.
Collins, J.F., Hu, Z, Ranganathan, P. N., Feng, D., Garrick, L.M., Garrick, M.D., Browne, R.W. (2008) Induction of Arachidonate 12-Lipoxygenase in Intestine of Fe-Deficient Rats Results in Production of Biologically Active Lipid Mediators. Am. J. Physiol. GI/Liv. Physiol. 294(4):G948-62.
Collins, J.F. (2007) Novel insights into intestinal and renal folate transport. Am. J. Physiol. Cell Physiol. Editorial Focus. 294(2):C381-2. Epub 2007 Dec 5.
Hu, Z., Hu, B., Collins, J.F. (2007) Prediction of synergistic transcription factors by function conservation. Genome Biology. 2007 Dec 5;8(12):R257 [Epub ahead of print].
Collins, J.F., Hu, Z. (2007) Promoter analysis of intestinal genes induced during iron-deprivation reveals enrichment of conserved Sp1-like binding sites. BMC Genomics. 2007 Nov 15;8(1):420 [Epub ahead of print].
Collins, J.F. (2006) Gene Chip Analyses Reveal Differential Genetic Responses to Iron-Deficiency in Rat Duodenum and Jejunum. Biol. Res.39:25-37.
Kolek, O.I., Hines, E.R., Jones, M.D., Lesueur, L.K., Lipko, M.A., Kiela, P.R., Collins, J.F., Haussler, M.R., and Ghishan, F.K. (2005) 1α,25-dihydroxyvitamin D3 up-regulates FGF23 gene expression in bone: the final link in a renal-gastrointestinal-skeletal axis that controls phosphate transport. Am. J. Physiol. Gastrointest. Liver Physiol. 289(6):G1036-42.
Ravia, J.J., Stephen, R.M., Ghishan, F.K., and Collins, J.F. (2005) Menkes Copper ATPase (Atp7a) is a novel Metal-Responsive Gene in Rat Duodenum and Immunoreactive Protein is Present on Brush-Border and Basolateral Membrane Domains. J Biol Chem. 280(43):36221-7.
Collins, J.F., Cumming, C. A., Kowdley, K. V., and Ghishan, F.K. (2005) Identification of Differentially Expressed Genes in Response to Dietary Iron- Deprivation in Rat Duodenum. Am. J. Physiol. Gastrointest. Liver Physiol. 288(5):G964-71, 2005.
Xu, H., Uno J.K., Inouye, M., Collins, J.F., and Ghishan, F.K. (2005) NF1 transcriptional factor(s) is required for basal promoter activation of the human intestinal NaPi-IIb cotransporter gene. Am. J. Physiol. Gastrointest. Liver Physiol. 288(2):G175-81.
Dixit, M.P., Xu, L., Xu, H., Bai, L., Collins, J.F., and Ghishan, F.K. (2004) Effect of angiotensin-II on renal Na+/H+ exchanger-NHE3 and NHE2. Biochim. Biophys. Acta. 1664(1):38-44.
Xu, L., Dixit, M.P., Chen, R., Dixit, N.M., Collins, J.F., and Ghishan, F.K. (2004) Effects of angiotensin II on NaPi-IIa co-transporter. Biochim. Biophys. Acta. 1667(2):114-21.
Hines, E.R., Kolek, O., Serey, S., Jurutka, P., Haussler, M.R., Collins, J.F. and Ghishan, F.K. (2004) Vitamin D3 downregulates PHEX gene expression by apparent repression of a novel 110 kDa transactivating factor which interacts with a poly-adenine region in the promoter. J. Biol. Chem. 5;279(45):46406-14.
Collins, J.F., and Ghishan, F.K. (2004) Genetic responses to dietary phosphorus deprivation: lessons learned from the rainbow trout. Am. J. Physiol. Regul. Integr. Comp. Physiol. Editorial Focus. Am. J. Physiol. Regul. Integr. Comp. Physiol. 287(3):R522-3.
Kiela, P.R., LeSueur, J., Collins, J.F. and Ghishan, F.K. (2003) Transcriptional regulation of the rat NHE3 gene: Functional interactions between GATA-5 and Sp family transcription factors. J. Biol. Chem. 278(8):5659-68.
REVIEW ARTICLES AND CHAPTERS
Collins, J.F. Bai, L., Xu, H., and Ghishan, F.K. (2005) Chapter entitled “Molecular Aspects and Regulation of Gastrointestinal Function During Post-Natal Development”. For Physiology of the Gastrointestinal Tract, 4th Edition. Academic Press.
Collins, J.F. and Ghishan, F.K. (2005) Chapter entitled “Molecular Mechanisms of Intestinal Transport of Calcium, Phosphate and Magnesium”. For Physiology of the Gastrointestinal Tract, 4th Edition. Academic Press.
Collins, J.F., Bai, L., and Ghishan, F.K. (2003) The SLC20 family of proteins: dual functions as sodium-phosphate cotransporters and viral receptors. Review. Pflugers Archives (European Journal of Physiology). 447(5):647-52.
Contact Information
359 Food Science and Human Nutrition Building
P.O. BOX 110370
Newell Drive
University of Florida
Gainesville, Fl 32611-0370
Phone:(352) 392-1991, ext. 216
Email: jfcollins@ufl.edu
Website: http://fshn.ifas.ufl.edu/faculty/jfcollins/
