James F. Collins, Ph.D., Associate Professor

CollinsTeam

Collins team (2010), left to right:

Sukru Gulec, Ph.D.; Dr. Collins; April Kim, M.S.; Liwei Xie, Ph.D.1; Lingli Jiang, Ph.D.; Yan Lu, Ph.D.2; Paul Fine, P.N.; (Raghu) Ranganathan, Ph.D.

1Currently a postdoctoral researcher in the Department of Physiology, Univ. of Michigan

2Currently a postdoctoral researcher at the Queensland Institute of Biomedical Research, Brisbane, Australia

Background

Dr. Collins has broad training in biology, physiology and nutrition, and has been involved in biomedical research for over 15 years, including post-doctoral work in transport physiology at the University of Arizona. His past studies focused on molecular aspects of intestinal sodium and phosphate absorption, but more recent investigations relate to iron metabolism. This is an important area of research, as body iron, unlike most other nutrients, is controlled at the level of absorption since no active excretory systems exist in mammals. Studies over the past 5+ years have identified copper-dependent aspects of iron transport and ongoing studies seek to further elucidate how copper influences body iron homeostasis. More recent experiments have identified an unknown ferroxidase in mammalian enterocytes and in-progress studies are designed to identify this novel protein and to elucidate its role in iron transport. Commonly used experimental methodologies include many molecular biology applications, such as qRT-PCR and western blotting, and techniques to study gene transcription (e.g. promoter analyses, ChIP assays). The Collins laboratory is staffed predominantly by Nutritional Sciences doctoral students, four of which have graduated in the past year.

 

Current Grant Support

1R01 DK074867 (Collins, PI); Title: Molecular Mechanisms of Intestinal Metal Ion Transport During Iron-Deficiency. 3/1/13-2/28/17; Sponsor: National Institute of Digestive, Diabetes and Kidney Diseases (NIDDK); $1,300,000 (total costs).

 

Past Grant Support

1R01 DK074867 (Collins, PI); Title: Molecular Mechanisms of Intestinal Metal Ion Transport During Iron-Deficiency; 7/1/07-6/30/12; Sponsor: National Institute of Digestive, Diabetes and Kidney Diseases (NIDDK); $1,468,461 (total costs).

1 R21 DK068349 (Collins, PI); Title: Intestinal Iron Transport in Iron Deficiency Anemia.NIH, NIDDK; 7/1/04-6/30/07 Sponsor: National Institute of Digestive, Diabetes and Kidney Diseases (NIDDK); $250,000 (direct costs).

 

Professional Affiliations

  • International Society for Trace Element Research in Humans (ISTERH) [2007]
  • East Coast Iron Club (2005; Chair, 2010-2013)
  • International BioIron Society (2004)
  • American Physiological Society (1999)
  • American Gastroenterological Association (1998)

 

Advisory Group Service

Scientitic Merit Review Subcommittee for Gastroenterology (standing member); Department of Veterans Affairs, Veterans Health Administration, Washington D.C., June 2013-June 2017.

Clinical and Integrative Molecular Gastroenterology study section (standing member); National Institute of Digestive, Digestive and Kidney Diseases (NIDDK), Washington D.C.; July 2012- May 2018.

 

Editorial Board Member

Journal of Nutrition (2010-present)

PLOS One (2013-present)

 

Faculty Links

 

Recent Peer-Reviewed Papers (out of >60 total)

Xie, L., Collins, J.F. (2013) Transcription Factors Sp1 and Hif2a Mediate Induction of the Copper-Transporting ATPase (Atp7a) Gene in Intestinal Epithel;ial Cells During Hypoxia. J. Biol. Chem. doi:10.1074/jbc.M113.489500 (published online June 28, 2013)

Gulec, S., Collins, J.F. (2013) Investigation of Iron Metabolism in Mice Expressing a Mutant Menke’s Copper Transporting ATPase (Atp7a) Protein with Diminished Activity (Brindled; MoBr/y). PLOS ONE. 11;8(6):e66010. doi: 10.1371/journal.pone.0066010

Xie, L., Collins, J.F. (2012) Copper stabilizes the Menkes copper-transporting ATPase (Atp7a) protein in rat intestinal epithelial cells. Am. J. Physiol. Cell Physiol., 304(3):C257-62

Lu, Y., Kim, C., Collins J.F. (2012) Multiple Menkes copper ATPase (Atp7a) transcript and protein variants are induced by iron deficiency in rat duodenal enterocytes. J. Trace Elem. Biol. Med. 26(2-3):109-14

Ranganathan, P.N., Lu, Y., Fuqua, B.K., Collins, J.F. (2012) Discovery of a cytosolic/soluble ferroxidase in rodent enterocytes. Proc. Natl. Acad. Sci. USA. 109(9):3564-9.

Ranganathan, P.N., Lu, Y., Fuqua, B.K., Collins, J.F. (2012) Immunoreactive Hephaestin and ferroxidase activity are present in the cytosolic fraction of rat enterocytes. Biometals. 25(4):687-95.

Klevay, L., Collins, J.F. (2011) Copper. Adv. Nutr. 2(6):520-22.

Jiang, L, Ranganathan, P.N., Lu, Y., Kim, C., Collins J.F. (2011) Exploration of the Copper Related Compensatory Response in the Belgrade Rat Model of Genetic Iron Deficiency. Am. J. Physiol. Gastrointest. Liver Physiol. 301(5):G877-86.

Ranganathan, P.N., Lu, Y., Jiang, L., Kim, C. Collins, J.F. (2011) Serum ceruloplasmin protein expression and activity increases in iron-deficient rats and is further enhanced by higher dietary copper intake. Blood. 118(11):3146-53.

Xie, L., Collins, J.F. (2011) Transcriptional regulation of the Menkes copper ATPase (Atp7a) gene by hypoxia-inducible factor (HIF2α) in intestinal epithelial cells. Am. J. Physiol. Cell Physiol. 300(6):C1298-305.

Hu, Zihua, Gulec, S., Collins, J.F. (2010) Cross-Species Comparison of Genome-Wide Gene Expression Profiles Reveals a Preferential Induction of Hypoxia Inducible Factor (HIF) Responsive Genes in Iron Deprived Intestinal Epithelial Cells. Am. J. Physiol. Cell Physiol. 299:930-938.

Collins, J.F., Prohaska, J.R., Knutson, M.K. (2010) Metabolic Crossroads of Iron and Copper. Nutr. Rev.68(3):133–147.

Collins J.F., Hua, P., Lu, Y., Ranganathan, P.N. (2009) Alternative Splicing of the Menkes Copper Atpase (Atp7a) Transcript in the Rat Intestinal Epithelium. Am. J. Physiol. Gastrointest. Liver Physiol. 297: G695–G707.

 

Dr. Collins Contact Info: Phone: (352) 392-1991, ext. 289; Email: jfcollins@ufl.edu

Website: http://fshn.ifas.ufl.edu/james-f-collins/