Lectures

The following lectures are presented in PowerPoint format. Lectures will be linked on the Friday before they will be presented to the students.

• Lecture Outline - Part 1

  • TOPIC	Relative Dates	Lecture Presentations
    Introduction, Questionnaire	May 14	Lecture 1 Handout Questionnaire
    Lab Safety	May 14	Lecture 2 Handout
    Sample Prep – Drying and Grinding, Filtration, Concentration, etc.	May 14	Lecture 3 Handout Filtration Handout
    Measuring – Accuracy and Precision, Errors and Error Propagation, Units	May 16	Lecture 4 Handout
    GLP (Good Laboratory Practices) Quality Assurance/Quality Control	May 21	Lecture 5
    Introduction to the Chemistry of Separation	May 23	Lecture 6 Homework
    Solvents - UV Cutoff, Background Signals, Viscosity, Miscibility, Instability	May 28	Lecture7 Handout Handout Solvent Handout
    Solvents - Solvent Strength, Polarity, Mixing Solvents	May 28
    Spectroscopy – Infrared, Nuclear Magnetic Resonance, UV/Vis	May 30	Lecture 8 Handout Spectro Handout NMR Tutorial
    Spectroscopy - Transmittance and Absorbance, Absorbing Species	May 30	Lecture 9 Handout
    Spectroscopy - Beer’s Law, Instruments - UV/VIS, Fluorescence	June 4
    Intro to Chromatography - General Description, Fundamental Equations Describing Separation, Zone Broadening and Column Efficiency	June 6	Lecture 10 Handout
    EXAM	June 6

• Lecture Outline - Part 2

  • TOPIC	Relative Dates	Lecture Presentations
    Chromatography - Linearity, Sensitivity, Optimization of Column Performance, Quantification	June 11	Lecture 11 Handout
    Derivatization in Chromatographic Analysis	June 13	Lecture 12
    Column Chromatography, Thin Layer, Size Exclusion, Chiral	June 18	Lecture 13 Handout
    HPLC – Introduction, Instruments – Pumps, Injectors, Mobile Phase Handling, Tubing, etc.	June 20	Lecture 14 Handout
    SUMMER BREAK	June 24-28
    HPLC - Detectors - UV/VIS, Photodiode Array, Fluorescence, Refractive Index, Electrical Conductivity	July 2	Lecture 15 Handout
    HPLC continued - Capillary Electrophoresis	July 2	Lecture 16 Handout
    HPLC – Normal Phase, Reverse Phase	July 9	Lecture 17 Handout Lecture 18 Handout
    Chromatography of Ions    Paired Ion, Ion Exchange	July 11	Lecture 19 Handout
    EXAM	July 11

• Lecture Outline - Part 3

  • TOPIC	Relative Dates	Lecture Presentations
    HPLC – Care and Maintenance of Equipment, Quality Control, Troubleshooting	July 16	Lecture 20 Handout Phenomenex HPLC Guide
    Gas Chromatography Introduction	July 18	Lecture 21 Handout
    Gas Chromatography, Injection	July 23	Lecture 22 Handout
    Gas Chromatography Mobile and Stationary Phases, Plot columns	July 23	Lecture 23 Handout
    GC – Detection	July 23	Lecture 24 Handout
    GC – Detection	July 25	Lecture 25 Handout Phenomenex GC Guide
    GC -Care and Maintenance of Equipment, Quality Control, Troubleshooting	July 25	Lecture 26a
    Mass Spectrometry	July 30 - August 6	Lecture 26b
    EXAM	August 6